| 1. | The research on guhcl - induced protein denaturation 盐酸胍诱导的蛋白质变性现象研究 |
| 2. | Research on urea - induced protein denaturation 尿素诱导的蛋白质变性现象的研究 |
| 3. | Study on the relation between chilling resistance and low - temperature - induced proteins in cotton seeding 棉苗抗冷性与低温诱导蛋白关系的研究 |
| 4. | Homology searches using blast with the amino acid sequence revealed that pdipl shares a high degree of homology ( 70 % identity ) with the tnf - induced protein b12 此外,通过blast检索发现pdip1蛋白与一个受tnf -诱导的蛋白质b12十分相似,彼此的氨基酸序列高达70是相同的。 |
| 5. | To su mmarize the advances of the study on cold induced proteins and cold induced genes of plant in recent years , and discussed relation between the proteins and the genes and cold stress resistance of plant 摘要现就近年来植物低温诱导蛋白和低温诱导基因的研究进展进行了概述,同时探讨了低温诱导蛋白和低温诱导基因与植物抗寒能力之间的关系。 |
| 6. | Among them , 29 proteins are up - regulated and 18 ones down - regulated , while 12 proteins are inhibited . 25 induced proteins were identified by means of maldi - tof / ms . the results showed that these proteins were closely related to heat - sho ck proteins ( dnak , hsp60 ) Rt19在盐激后,会有84个差异蛋白出现,其中诱导表达的蛋白有25个,阻抑表达的蛋白有12个,表达量上调的蛋白有29个,表达量下调的蛋白点18个。 |
| 7. | The routine method for inducing protein function evolution is imitating and speeding protein gene mutation . in this research - amylase gene was mutated in vitro by pcr with dntp in which deoxythymidine triphosphate ( dttp ) was partially replaced by 5 - bromo - 2 ' - deoxyuridine - 5 ' - triphosphate ( 5 - bdu ) . the higher the concentration is , the stronger its inducing ability is in a certain range 本研究采用5 -溴脱氧尿苷三磷酸( 5 - bdu )部分取代脱氧胸苷三磷酸( dttp ) ,在pcr的过程中对克隆的野油菜黄单胞菌的-淀粉酶基因进行了体外诱变,诱变结果表明: 5 - bdu浓度越高,诱变越强;浓度越低,诱变越弱。 |
| 8. | About 25 salt - induced proteins were identified by maldi - tof / ms , and finally the function of 17 induced proteins were determined , including heat - shock proteins ( groes , clp ) . abc transporter , rna polymerase ( p - subunit ) and enzymes involved in signal transduction and metabolism . meanwhile , 84 proteins were idifferentially expressed after salt shock 5 min and 50 min in late exponential phase 应用maldi - tof ms对30个盐胁迫蛋白进行分析,已初步确定17个诱导蛋白的功能,其中包括与盐胁迫相关的热激蛋白( groes 、 clp ) 、与代谢途径和信号传导等有关的酶,以及abc转运蛋白、转录调节蛋白、 rna聚合酶的亚基等。 |
| 9. | Twenty - six induced proteins were analyzed by matrix assisted laser desorption / ionization time - of - flight mass spectrometry ( maldi - tof / ms ) and identified by database searching . among them , 20 ones were assigned and their functions were known , including salt stress proteins , or enzymes are related to salt stress , y - butyrobetaine and iipopolysaccharide synthesis , metabolism pathway and signal transduction 应用maldi - tof ms对26个盐激诱导蛋白进行分析,将获得的肽指纹图谱经数据库检索,初步确定了20个诱导蛋白的功能,其中包括与盐胁迫、 -丁酰甜菜碱、脂多糖合成、代谢途径和信号传导等有关的酶。 |